Effects of CXCL12 on GABA and ACh secretion and its mechanism in cultured hippocampal cells

来源 :中国神经科学学会第九届全国学术会议暨第五届会员代表大会 | 被引量 : 0次 | 上传用户:ywanywhere
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  Objective This study is to illustrate the effects of CXCL12 on GABA and ACh secretion and its mechanisms.Methods Primary hippocampus neurons were obtained from newborn SD rats.Bilateral hippocampi were carefully dissected and digested in 0.25% trypsin at 37 ℃ for 5 min.Immunocytochemistry were used to determine the location of CXCL12 and its receptor CXCR4.And ELISA was carried to detect the dose of CXCL12 in primary cultures.In addition, GABA and ACh secretion were detected by ELISA under different culture condition, including AMD3100 and CXCL12 stimulation.Results CXCL12 and its receptor CXCR4 mainly located in membrane and cytoplasma, which suggested the role of CXCL12 and its receptor in nervous system.In addition, CXCL12 secretion increased significantly with culture time extension.Moreover, CXCL12 could stimulate ACh secretion and inhibit GABA secretion.But when CXCR4 was knocked down by AMD3100, ACh and GABA secretion changed sharply in an opposite direction.Conclusion CXCL12 may alter GABA and ACh secretion by CXCR4 pathway in cultured hippocampal cells, which may suggest CXCL12 as an important neurotrophic factor to stimulate neurotransmitters releasing.
其他文献
作为传统无线通信技术体系的重要组成部分,短波通信技术的出现解决了远距离通信质量问题,为船舶通信距离的延展奠定了技术基础.然而,由于短波通信同属于以电磁波为载体的信息
在牧区小学数学教学中,培养学生创新意识是势在必行的,我们必须突破重重难关,落实任务,为牧区学生的全面发展而服务.本文结合牧区实际教学情况,分析了牧区小学数学教学中培养
比利时一家报社的记者对甘培安有这样的描述:“在安特卫普有一位活跃的华人社会活动家,他时而穿牛仔驾雷诺(小型客货两用车);有时又穿西装驾着顶级丹拿车。”甘培安看后哈哈
针对某型直升机全机振动测试的试飞任务,分析了测试需求,完成了测试系统的设计,实现了测试系统的建立,进行了装机环境下的振动标定和飞行试验中的数据采集,对数据进行分析后,
目的了解西安地区流行性腮腺炎的流行特征,为预测疾病发病趋势、预防和控制疾病提供科学依据。方法采用描述流行病学分析方法对西安市流行性腮腺炎病例进行分析。结果西安市2
  Hippocampus is one of the important sites for mammalian learning and memory, and Long-term potentiation (LTP) is a potential neurophysiological mechanism re
会议
经典应成为学生学习的需要,使之进入学生的心灵之中,活用于学生的习作之中,使经典不断焕发时代的气息.
化学是一门实验学科,其中的很多知识点都是通过实验能够验证或者得出结论的.因此,在化学教学中,教师应该对实验教学加以重视,通过增加演示实验、分析典型实验、设计贴近生活
某同志购置了一台日本皇冠35厘米黑白电视机,为提高收看效果,在室外架设了一个很高的接收天线。将300Q馈线引入室内,并接到一个代替开关使用的电源插座上。遇到雷雨天,就把
矿山开采的过程具有一定的严峻性以及复杂性.故而矿山机械设备的使用过程中,由于施工条件较为恶劣,设备发生故障的频率明显较高.针对该种情况,应建立起相应的科学管理手段以