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Objective To clarify the anatomy of the first toe webbing space for harvest of the second toe in thumb reconstruction.Methods The first dorsal metatarsal artery,the first deep transverse metatarsal ligament and the extensor expansion were observed on 42 adult cadaveric lower extremities.Clinically the method of tracing the first dorsal metatarsal artery around the space of the extensor expansion was used in 36 cases of thumbs reconstruction.