【摘 要】
：
Sugarcane variety ROC22 was used as the experimental material and water stress treatment was performed for the 4 or 5-leafed plants with 25%polyethylene glycol(PEG)6000 solutions.The cDNA sequence of
【机 构】
：
Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Guangxi Academy of Agricultural Scienc
【出 处】
：
广西甘蔗学会2016年年会暨学术交流会
论文部分内容阅读
Sugarcane variety ROC22 was used as the experimental material and water stress treatment was performed for the 4 or 5-leafed plants with 25%polyethylene glycol(PEG)6000 solutions.The cDNA sequence of ScP5CS gene in sugarcane with 2151 bp in length was isolated by homologous cloning,which contained an open reading frame encoding a protein of 716 amino acids,with GenBank accession number EU005373.Comparing the sequence of ScP5CS with that of sugarcane P5CS reported in GenBank,the nucleotide acid showed high identity(98%),but the deduced amino acid was only 92%.The deduced protein contains putative ATP-binding site,putative leucine domains,Glu-5-kinase domain,putative NADPH-binding domain,conserved GSA-DH domain and feedback inhibition site.Besides,there were more differences in Glu-5-kinase domain from the deduced amino acid of sugarcane P5CS reported(ABM30223),but less for the P5CSs from rice(Oryza sativa)and wheat(Triticum aestivum).So it is confirmed this gene is a new gene of sugarcane P5CS.
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