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目的 探讨芍药甙对胃肠平滑肌细胞的作用及其发挥作用的机制和信号转导途径。方法 采用平滑肌细胞长度测定和激光扫描共聚焦显微镜细胞内游离钙离子浓度 ([Ca2 + ]i)测定技术 ,观测芍药甙对胃肠平滑肌细胞的平均长度的变化 ;选择性PKA抑制剂和选择性PKG抑制剂对芍药甙舒张平
Objective To investigate the effect of Paeoniflorin on gastrointestinal smooth muscle cells and the mechanism and signal transduction pathway. Methods The length of gastrointestinal smooth muscle cells was measured by smooth muscle cell length measurement and laser scanning confocal microscopy intracellular free calcium ion concentration ([Ca2 +] i) measurement technique. Selective PKA inhibitor and selectivity PKG inhibitors against peony