【摘 要】
：
目的：检测多发性硬化(MS)患者外周血淋巴细胞(PBMC)中辅助性T细胞17 (Th 1 7)特异性转录因子维甲酸相关核孤儿受体γ t(RORγ t)、B细胞转录激活因子(BATF)及外周血中Th17细胞相关细胞因子白细胞介素10(IL-1O)、白细胞介素17(IL-17)和白细胞介素27(IL-27)表达水平,探讨其在MS发病中的作用.方法：以缓解-复发型多发性硬化急性发作期患者及健康对照组各5
【机 构】
：
齐齐哈尔医学院附属第三医院神经内科,齐齐哈尔,161002 哈尔滨二四二医院神经内科,哈尔滨,15
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目的：检测多发性硬化(MS)患者外周血淋巴细胞(PBMC)中辅助性T细胞17 (Th 1 7)特异性转录因子维甲酸相关核孤儿受体γ t(RORγ t)、B细胞转录激活因子(BATF)及外周血中Th17细胞相关细胞因子白细胞介素10(IL-1O)、白细胞介素17(IL-17)和白细胞介素27(IL-27)表达水平,探讨其在MS发病中的作用.方法：以缓解-复发型多发性硬化急性发作期患者及健康对照组各53例为研究对象,抽取MS患者治疗前及治疗4周后、健康人的空腹静脉血.ELISA法测定血清中IL-10、IL-17和IL-27蛋白表达水平,流式细胞术检测PBMC中Th17细胞(CD4+IL-17+细胞)的百分率,实时荧光定量PCR(RT-PCR)法检测PBMC中BATF和RORγ tmRNA表达水平.结果：治疗前与健康对照组比较,血浆IL-17水平较高,而IL-10、IL-27水平均较低,外周血PBMC中BATF和RORγ t mRNA表达水平均升高,Th17细胞(CD4+IL-17+细胞)百分率明显升高(均P＜0.05)；治疗前、后比较,血浆IL-17水平降低,相反IL-I0、IL-27水平均升高,BATF和RORY tmRNA表达水平均降低,Th17细胞(CD4+IL-17+细胞)百分率明显降低(均P＜0.05)；治疗后与健康对照组比较,IL-27具有统计学意义BATF和RORγ tmRNA表达水平均升高,Th17细胞(CD4+IL-17+细胞)百分率明显升高(均P＜0.05).结论：Th17转录因子RORγ t和BATF及Th17细胞及其相关细胞因子IL-10、IL-17和IL-27参与了MS的发生与进展.
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