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Objective: To evaluate the consistency of manual and automated measurements of monodominant follicles with different follicle size in infertile patients.Methods: Transvaginal two-dimensional (2D) ultrasound and SonoAVC (Sonography-based Automated Volume Calculation) were performed in 226infertile patients with monodominant follicle growth.2D diameters were separately compared with SonoAVC-generated d(V) and m-d values in different follicle category,i.e.>10 to 14 mm,>14 to 18 mm,>18 to 22 mm and >22 mm.Results: There was moderate degree of consistency between 2D diameter and SonoAVC-generated parameters regardless of follicle size.The mean difference was 0.82 mm between 2D diameter and SonoAVC-generated d(V) value,and 0.22 mm between 2D diameter and SonoAVC-generated m-d value,respectively.The discrepancy of manual and automated measurements tended to increase as follicle size increased.Conclusion: Compared with manual measurement,SonoAVC might underestimate follicle size.The absolute size of a follicle affected the consistency of two techniques.