【摘 要】
：
A capillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) detection method was developed to detect doxorubicin (DOX) uptake in single K
【机 构】
：
School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China
【出 处】
：
第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
论文部分内容阅读
A capillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) detection method was developed to detect doxorubicin (DOX) uptake in single K562 cells based on drug dosage and exposure time.The K562 cells were treated with different concentration of DOX (1, 3, 10, 20, 30 and 50 μM) for different exposure time (1, 3, 5, 24 and 48 h), respectively.A minimum of 20 cells were analyzed for each DOX-treated cell population.The marked heterogeneity in DOX uptake was observed and the variation of DOX uptake amount (RSDs %) ranged from 24.0 % to 61.1% within cell populations.The cell-to-cell variation in DOX uptake decreases and tends to be constant with increasing DOX concentration, but does not exhibit regular variation with increasing exposure time.The mean intracellular DOX amount is a linear function of extracellular DOX concentration.
其他文献
Amino acids (AAs) are very important in the fields of biology and biochemistry.As the building blocks of proteins and bioactive composition in various biosy
Although GC/TOF systems have been applied to a variety of quantitative and qualitative tasks for target analytes, non-targets and true unknowns (no referenc
在3年前,我们开始思考一个问题:既然出现了恶化的物流配送,那么供应链管理该如何去做?这也是中国零售企业在追求规模扩张到精细化管理时所要思考的一个供应链问题。
Three y
公司日前出其不意出台了股权激励方案,此次激励的利好主要体现在:稳定中高层管理团队;稳定投资者对公司未来业绩增长的预期、提升估值;形成各方利益的高度一致。上调苏宁目标
请下载后查看，本文暂不支持在线获取查看简介。
Please download to view, this article does not support online access to view profile.
声卡、音箱、耳机、视频采集卡、视频转换器等多媒体设备方面,摄像头、视频采集卡等多媒体设备已经从专业领域走向平民化。但是由于移动数码产品的冲击,其表现并不如人意,只
湖南省南县饮服公司摄影社,在有关单位的支持下,制成一台室内摄影照明遥控装置。这台装置性能良好,操作简便,使用安全,节省时间并且提高了服务质量。一、原理:由发射装置产
Coinage metals have got considerable attention in recent years because of their "noble" characteristics with respect to their relativistic effect and releva
应用时域的傅里叶方法,求出脉冲响应近似为正弦平方脉冲的五极、七极和九极网络的传递函数,其近似精度比七极汤姆逊网络更高。七极汤姆逊网络的脉冲响应曲线与正弦平方曲线的