To prepare antioxidative peptides from venison protein hydrolysates (APVPH),venison was hydrolyzed six proteinase such as pepsin,trypsin,papain,tt-chymotrypsin,Alcalase and Neutrase,and the antioxidative activities of their hydrolysates were investigated using free radical scavenging assay by electron spin resonance,spin-trapping technique.Among hydrolysates,papain hydrolysates having the highest antioxidative activity,further separated into four groups using ultrafiltration membranes and purified consecutive chromatographic methods.