In Vivo Knockdown of the Androgen Receptor Results in Regression and Growth Inhibition of Androgen-i

来源 :BIT Life Sciences 1st Annual World Cancer Congess-2008(2008中 | 被引量 : 0次 | 上传用户:wenruozhu
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  Progression to the castration resistant, androgen independent state is the incurable and lethal end stage of prostate cancer, where the tumors are no longer dependent on circulating androgens for growth.However, there is strong evidence that the androgen receptor (AR), the protein that mediates androgen actions, still plays a central role in this process.In a previous report (Cheng et al., Cancer Res, 2006), we used an antibiotic (doxycyeline)-inducible short hairpin RNA (shRNA) to knockdown the AR in androgen-dependent LNCaP prostate cancer tumor xenografts in immunocompromised mice and found that we could decrease serum PSA to well below castration nadir levels and could significantly retard tumor growth over the entire 55-day experimental period, with no evidence of progression to androgen independence.This was the first demonstration that in vivo knockdown of AR could delay or prevent progression to androgen independence.In the present study, we tested whether knockdown of the AR in an androgen-independent human prostate cancer (C4-2 cells) could affect PSA secretion and tumor growth.Changes in serum PSA levels and tumor volumes after induction ofAR shRNA fell into two subgroups based on their responses.
其他文献
Hsp70 is known to constitute the ubiquitous cellular protective mechanism whose efficacy was proved in a great number of studies in vitro and in vivo.In cancer cells Hsp70-mediated protection is mainl
会议
Breast cancer is a common but biologically diverse group of malignancy, with considerable heterogeneity in behavior, outcome and response to therapy.Recent gene expression profile studies have led to
会议
Skeletal metastasis is a major complication of advanced breast cancer which results in bone fractures and considerable pain burden.Tumor cells arriving at bone metastatic sites disrupt the balance bet
会议
The c-Jun NH2-terminal kinases (JNKs) are members of the mitogen-activated protein kinase family and active several transcription factors, including p53, c-Jun and activating-protein 1.Thus, the JNK s
会议
Recurrence of carcinomas due to cells that migrate away from the primary tumor is a major problem in cancer treatment and a cause of cancer recurrence.Fascin is an actin-bundling protein that is low o
会议
Proton magnetic resonance imaging (MRI), with or without contrast enhancement, has emerged in recent years as the preferred noninvasive neuroimaging modality for clinical evaluation and monitoring the
会议
Brain tumors are now the leading cause of cancer-related deaths in children under age 15.Malignant gliomas are, for all practical purposes, incurable and new therapeutic approaches are desperately nee
会议
The molecular signature of chronic myeloid leukemia (CML) is the BCR-ABL fusion gene originating in a pluripotent hematopoietic stem cell.The BCR-ABL oncoprotein (p210BCR-ABL) has constitutively eleva
会议
With current treatment protocols more than 85% of adults with acute lymphoblastic leukemia (ALL) achieve complete remission (CR), however, approximately halfofCR patients subsequently relapse, which i
会议
Compelling evidences indicate a key role for regulatory T cells (Tregs) on the host response to cancer and recent studies indicated that the generation of effective WTl-specific cytotoxic T cells can
会议