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Background: Aberrant methylation of cytosine-guanine dinucleotide (CpG) islands is one of the most common epigenetic modifications involved in the development of urothelial carcinoma.However, it is unknown that the predictive role of methylation to contralateral new upper tract urothelial carcinoma (UTUC) after radical nephroureterectomy(RNU).Objective: To evaluate the role of methylation of ten genes in predicting contralateral UTUC recurrence after RNU.