The present study was conducted to investigate the growth performance,lipid deposition,lipid peroxidation and whole body fatty acids of Russian sturgeon fed with graded levels of dietary lipids (5 %,15 % and 25 %) with different n-3/n-6 ratios (1:3,1:1 and 3:1).After 8 weeks feeding,the groups of fish fed high lipid (25 % lipid) showed higher growth and lower FC than fish fed lower dietary lipid (15 % and 5 % lipid).HSI increased with dietary lipid level,but VSI was not affected.The fat content of whole body,muscle and liver were significantly increased in Russian sturgeon fed 25 % lipid,and fat content of liver was much higher than that of whole body and muscle indicated that liver is the main fat deposition site of Russian sturgeon.Our results showed that the serum CHO was not affected,but the serum TG and GLU were significantly affected.Lipid peroxidation were elevated with the increased dietary lipid level and n-3/n-6 ratio.Increased serum GOT and GPT were found in L25 groups,and they were significantly elevated in L25 3:1 group.Russian sturgeon fed L25 3:1 diet had the highest lipid peroxidation capacity.In whole body,both saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) percentages were significantly increased,while the percentage of polyunsaturated fatty acid (PUFA),including n-3 PUFA,n-6 PUFA,EPA+DHA decreased in 25 %-fat group.In summary,our finding suggested that Russian sturgeon can utilize high lipid diets and fat mainly deposited in liver.Moreover,higher n-3/n-6 ratio may increase lipid peroxidation and resulted in serious adverse effects,such as fatty liver.