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To construct fusants through protoplast fusion for enhancing imazethapyr and chiorimuron-ethyl degradation simultaneously,intergeneric protoplast fusion between Beijerinckia sp.P310-1 and Rhodococcus sp.D310-1 was firstly performed and confirmed.Subsequently,the degradation capacity of fusant R3 to imazethapyr and chiorimuron-ethyl were compared with the parents.Five fusants were obtained from the selective medium at the fusion frequency approximately 2.03×10-7.