Encapsulation of materials,their intracellular delivery and subsequent release can be performed using polyelectrolyte multilayer microcapsules.The presentation outlines encapsulation and release strategies for polymeric microcapsules.To-date,several encapsulation methods have been developed,while release from microcapsules has been also conducted by both chemical and physical methods,including pH,salt,ultrasound,magnetic particles and laser-nanoparticle interaction.The latter method is well-suited for cellular culture; the most important aspect of this research approach includes nanoparticle distribution control.Also,nanoparticles on microcapsules have been shown to improve mechanical properties of microcapsules-this and other approaches in designing microcapsules suitable for intracellular drug delivery are outlines.Initial examples of application of encapsulation and intracellular delivery using microcapsules have been conducted for cancer cells.Recent work included tracking peptide-MHC Class Ⅰ complex presentation in immuno-cells-the results of this work are of significance to immunology and immunoresponse.Peptide-complex presentation has been observed in real-time,quantification of peptide presentation is performed analyzing fluorescent signal from the cell surface.Further perspectives on application of intracellular delivery are presented.