【摘 要】
：
PCR-RFLP technique was applied to distinguish mitten crab samples.The around 1600bp fragments of mitochondrial 12s-16s rDNA of mitten crab from ten areas in China (E.sinensis and E.japonicahepuensis)
【机 构】
：
Department of fisheries science, Tianjin Agricultural College, Tianjin, China, 300384
【出 处】
：
2005 World DNA and Genome Day(2005中国(大连)国际DNA和基因组节大会)
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PCR-RFLP technique was applied to distinguish mitten crab samples.The around 1600bp fragments of mitochondrial 12s-16s rDNA of mitten crab from ten areas in China (E.sinensis and E.japonicahepuensis) and one area in Russia (E.japonica) were amplified by using PCR technique.Twelve restriction endonucleases,Dra I,Hind Ⅲ,Hae Ⅲ,Hinf Ⅰ,Taq Ⅰ,Rsa Ⅰ,Hpa Ⅱ,Msp Ⅰ,BstB Ⅰ,Xba Ⅰ,EcoR Ⅰ,and EcoR V were used to digest the PCR products.In the mitochondrial 12s-16s rDNA fragments,there were some DNA sequences that could be recognized by the first six enzymes,namely,Dra Ⅰ,Hind Ⅲ,Hae Ⅲ,Hinf Ⅰ,Taq Ⅰ and Rsa Ⅰ,and among the 6 enzymes,Dra Ⅰ and Hind Ⅲ were polymorphic.The RFLP patterns showed that there were 4 composite restriction morphs,AAAAAA,BBAAAA,CCAAAA and BDAAAA.
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