Fungal Immunomodulatory Protein (FIP), extracted from higher Basidiomycetes, is a kind of small molecule protein with the extensive biological function, including the anti-tumor and anti-allergy, stimulating immune cells to produce a variety of cytokines, etc.In this study, FIP-SN15, a novel gene generated from the genes of Ganoderma sinensis and Ganoderma lucidum fungal immunomodulatory protein based on the DNA shuffling technique, was used as the object of this study.