【摘 要】
：
The initial stage of atherosclerosis is characterized by recruitment of leukocytes to activated endothelial cells (ECs).MicroRNAs (miRNAs) are a class of 19-25 nucleotides, non-protein-coding RNAs tha
【机 构】
：
Department of Cardiology,Changhai Hospital,Second Military Medical University,168 Changhai Road,Shan
论文部分内容阅读
The initial stage of atherosclerosis is characterized by recruitment of leukocytes to activated endothelial cells (ECs).MicroRNAs (miRNAs) are a class of 19-25 nucleotides, non-protein-coding RNAs that repress target gene expression by translational inhibition or mRNA degradation.The link between miRNA and endothelial functions is largely unknown.Northern blot showed that miR-155 and miR-221 were highly expressed in human umbilical vein endothelial cells (HUVECs) and vascular smooth muscle cells (VSMCs).Bioinformatics analysis proposed Ets1,a key endothelial transcription factor for inflammation and tube formation, as a candidate target for miR-155 and miR-221/222 cluster.
其他文献
目的:系膜增殖性肾炎是我国发病率最高的肾小球肾炎,目前机制不清.基质金属蛋白酶的组织抑制剂1(TIMP1)具有调控细胞外基质积聚、细胞增殖的作用,但是其对系膜细胞增殖的作用,目前尚不清楚.抗T hy-1 肾炎是经典的系膜增殖性肾炎模型,其具有典型的系膜细胞增殖和细胞外基质积聚.在前期的基因芯片结果中,我们发现TIMP1 在抗Thy1 肾炎模型的第3 天、第5 天、第7 天显著上调,提示TIMP1
背景与目的:慢性肾脏病(Chronic kidney disease,CKD)已成为全球公共的健康问题,但是目前尚缺乏特异性的治疗.肠道吸附剂和益生菌疗法显示出治疗CKD 的疗效,结肠渐成为治疗CKD 的新靶点.大黄复方灌肠在中国被广泛应用于治疗CKD 患者,且既往研究报告表明该疗法在一定程度上延缓疾病的进展.
Objective To investigate MCP-1-2518 G/A gene ploymorphisms action mechanism in coronary heant disease(CHD) patients.Methods The 360 patients were divided into 3 groups to the Coronary Angiography (CAG
研究背景 研究胡椒碱对H2O2引起的单个家兔心房肌细胞瞬时外向钾电流(Ito)异常的保护作用.方法 家兔心房肌细胞的分离:取兔(约1.0～1.5 kg)经腹腔20％水合氯醛(2ml/kg)麻醉,迅速取心脏,在37℃和通氧条件下行Langendorff灌流.用无Ca2+ Tyrode s液灌流3 ～ 5min,用含Ⅱ型胶原酶70 mg、胰蛋白酶12 mg无Ca2+Tyrodes液灌流(50 ml)
桹 bjective The differentiated function of Endothelial progenitor cells (EPCs) may play an pivotal roles in endothelial repair and reendothelialization replacement of postinjury endothelium.However, th
研究背景 研究胡椒碱对H2O2引起的单个家兔心房肌细胞动作电位时程(APD)及L型钙电流ICa,L异常的保护作用.方法 家兔心房肌细胞的分离:取兔(约1.0～ 1.5 kg)经腹腔20％水合氯醛(2ml/kg)麻醉,迅速取心脏,在37℃和通氧条件下行Langendorff灌流.用无Ca2+ Tyrodes液灌流3～5min,用含Ⅱ型胶原酶70 mg、胰蛋白酶12 mg无Ca2+ Tyrodes液灌
研究背景:利用低浓度过氧化氢(H2O2)建立兔原代左心房肌细胞氧化损伤模型,在此模型基础上研究胡椒碱对左心房肌细胞氧化损伤的保护作用.方法:将18只新西兰白兔随机分为3组:正常对照(normal control,NC)组,H2O2组和胡椒碱组,分别进行左心房肌细胞原代培养.
Atherosclerosis is one of the most common diseases in developed and developing countries.The proliferation of vascular smooth muscle cells (VSMCs) plays a crucial role in intimal thickening of arteria
Endothelial progenitor cells (EPCs) therapy represents a novel strategy for a variety of diseases.Interestingly, spleen acts an important reservoir during EPCs trafficking.Therefore, we investigate th
Endothelial cells are the key components of vascular intima and play pivotal roles in vasculogenesis, angiogenesis, and tumor growth.Using Northern blot and real-time PCR, we confirmed that miR-126 an