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Rapeseed (Brassica napus L.) is one of most important oilseed crops in the world.Seed oil content is always a significant character for rapeseed cultivars.However, little progress for elevating rapeseed oil content was acquired in recent years.In this study, we identified three high oil content (HO) lines (zy036, 6F313, and 61616) from different origins which seed oil content were all over 50％.