Cutting-edge synchrotron radiation-based analytical technology and new generation molecular biotechnology highly contribute to advances in development of new feeds and feed structure and ruminant nutrition research at cellular and molecular levels.The objective of this presentation is to briefly introduce two advanced technology that we are using for feed molecular structure in relation to nutrient utilization and availability in ruminants (dairy and beef cattle).The presentation includes two parts: Part Ⅰ is.: "Synchrotron as a Novel Tool for Feed Structure Research for Ruminant Cattle" and Part Ⅱ is "Bio-Feeds for Beef and Dairy Cattle" (Using New Generation Molecular Biotechnology to Develop New & High Quality Feeds, called "Bio-Feeds").The outline of our 1st Part includes the following topic: (1) What is a Synchrotron? Short Description-"Synchrotron & Photons"-Major Components of Synchrotron; (2) Synchrotron Molecular Spectroscopy Techniques-Advantage of Synchrotron Radiation and-Synchrotron Radiation (SR-IMS) Microspectroscopy; (3) Synchrotron Applications: Synchrotron-based Research Programs in our research team.The purpose of the 2nd Part of our research programs is to introduce gene-transformation technique to improve feed quality for cattle.This Part includes our two PhD programs: The 1st PhD project is about inserting single gene (Lc gene) to three varieties of winter-hardy alfalfa to modify Flavonoid Passway to make alfalfa produce new chemical compounds, Anthocyanidin and Proanthocyanidin (PA) or Polymeric Anthocyanidin in order to improve alfalfa Feeds Milk/Meat Value (FMV) and Reduce Bloat Incidence.The 2nd PhD project is about inserting double genes, triple genes and two genes to further modify Flavonoid Passway to improve the level of Anthocyanidin and Proanthocyanidin (PA) or Polymeric Anthocyanidinin.This project is still going on.Hope that our presentation will provide some insight as how to use available new advanced technology for feed and cattle research.