论文部分内容阅读
目的观察马尾松花粉水提物(PWE)对肥胖模型小鼠的体重、体脂肪和血脂的影响。方法选取60只昆明白雄性小鼠,体重(19±1)g,随机分为6组:空白对照组,高脂肥胖组,奥利司他组(0.05g/kg bw.d),PWE低、中、高(0.0625、0.125、0.25g/kg bw.d)剂量组。每三天测定一次体重,6w后检测血脂水平、脂肪因子和肉碱棕榈转移酶Ⅰ(CPT-Ⅰ)含量及CPT-ⅠmRNA的表达水平等。结果 PWE三个剂量均能降低小鼠体重和体脂总重,也能降低小鼠血清中TC、TG的含量,升高HDLC的含量,显著地升高肝脏组织和脂肪组织中CPT-Ⅰ的含量,PWE中剂量组尤为明显(P<0.01),显著上调CPT-ⅠmRNA的表达。结论 PWE早期应用能够有效的预防高脂饲料诱导的小鼠肥胖。[营养学报,2013,35(2):195-198]
Objective To observe the effects of Masson Pine pollen water extract (PWE) on body weight, body fat and lipids in obese mice. Methods Sixty Kunming white male mice weighing 19 ± 1 g were randomly divided into 6 groups: control group, hyperlipemia obesity group, orlistat group (0.05g / kg bw.d), low PWE , Medium and high (0.0625,0.125,0.25g / kg bw.d) dose group. The body weight was measured every three days. After 6 weeks, the levels of blood lipids, the levels of adipokines and carnitine palmitransferase Ⅰ (CPT-Ⅰ) and the expression of CPT-ⅠmRNA were determined. Results All three doses of PWE could reduce body weight and total body fat weight of mice, and also reduce the content of TC and TG in serum, increase the content of HDLC and significantly increase the content of CPT-Ⅰ in liver and adipose tissues (P <0.01), significantly up-regulated the expression of CPT-I mRNA. Conclusion Early application of PWE can effectively prevent obesity induced by high fat diet in mice. [Journal of Nutrition, 2013,35 (2): 195-198]