人胱抑素C的原核表达及活性鉴定

来源 :第十届全国检验与临床学术会议暨2015年中国医师协会检验医师年会 | 被引量 : 0次 | 上传用户：sammi696

【摘要】

：

在不改变氨基酸序列的前提下,对Cys C 的编码基因进行大肠杆菌同义密码子偏好性优化后,人工合成其序列,PCR 扩增Cys C 基因,其产物连接到pMD18-T 质粒,酶切并回收目的片段后克隆到表达载体pET-32a,然后将重组质粒pET-32a-CysC 转化大肠杆菌ER2566,大量表达后利用Westen Blotting 及ELISA 进行重组蛋白的活性及抗原性鉴定。

【作者】

：

李原蒋琰谌芳彭飞李桑吴意郑姣何赛刘如石

【机构】

：

湖南省人民医院检验科湖南师范大学生命科学学院

【出处】

：

第十届全国检验与临床学术会议暨2015年中国医师协会检验医师年会

【发表日期】

：

2015年3期

【关键词】

：

人胱抑素C 酶联免疫吸附试验(ELISA) 免疫反应性免疫原性

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人胱抑素C的原核表达及活性鉴定

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