Genetic Transformation With Ospgip1 Improves Disease-resistance of Brassica napus

来源 :第十九届国际十字花科遗传学会及芸薹属2014年年会 | 被引量 : 0次 | 上传用户:tianstone
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119 positive plants were obtained by Agrobacterium-mediated transformation of B.napus materials 7-5,P61-5 and T45,respectively.The T1 generation transgenic plants of Ospgip1 were confirmed by PCR,RT-PCR and Real-time PCR.The Sclerotinia inoculation test showed transgenic plants was performed disease-resistance.2000 positive plants from T1 generation were confirmed by PCR.PCR,RT-PCR and Real-time PCR analysis of the transgenic progenies showed that Ospgip1 were stably inherited and expressed.Coefficient (r=-0.72154) between the Ospgip1 expression and the plaque size was significant in T1 generation seedling leaves.
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