【摘 要】
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The red panda (Ailurus fulgens) is one of the flagship species in worldwide conservation.Though the international lineage of red pandas in captivity was established in 1980,management of the captive p
【机 构】
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The Key Laboratory for conservation biology of endangered wildlife,Sichuan Province Chengdu Research
【出 处】
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野生动物生态与资源保护第五届全国学术研讨会
论文部分内容阅读
The red panda (Ailurus fulgens) is one of the flagship species in worldwide conservation.Though the international lineage of red pandas in captivity was established in 1980,management of the captive population in China was haphazard,poorly documented,and characterized by frequent full-sib mating.These issues make the conservation and management of captive red pandas problematic.The microsatellite is a useful tool for determining parentage and evaluating population genetic diversity.So far,we characterized 25 microsatellite loci from two genomic DNA-enriched libraries of the red panda.The number of observed alleles among 35 samples of red pandas ranged from five to 17.Observed and expected heterozygosities were 0.286 to 0.971 and 0.121 – 0.909,and the mean polymorphic information content was 0.716.All loci followed Hardy-Weinberg expectations except two,which may due to the presence null alleles.Three pairs of loci exhibited significant linkage disequilibrium after Bonferroni correction for multiple comparisons.Then,we conducted the parent assignment of 32 red pandas at the Chengdu Research Base of Giant Panda Breeding (Sichuan,China).The results showed that all parents-offspring relationships are all confirmed.And there are high inbreeding coefficients in this population.
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