【摘 要】
：
温敏雄性不育(temperature sensitive male sterility,MS)在现代杂交水稻培育方面起着举足轻重的作用。为了探究植物温敏雄性不育的分子机制,我实验室通过EMS诱变野生型拟南芥Col-0,获得了一株温敏雄性不育突变体,该突变体表现为16℃可育,23℃半不育,27℃完全不育。通过图位克隆,发现该突变体突变基因为a tms1,为单碱基突变,其编码一个脂酰辅酶A合成酶。采用
【机 构】
：
上海大学生命学院,上海200444
论文部分内容阅读
温敏雄性不育(temperature sensitive male sterility,MS)在现代杂交水稻培育方面起着举足轻重的作用。为了探究植物温敏雄性不育的分子机制,我实验室通过EMS诱变野生型拟南芥Col-0,获得了一株温敏雄性不育突变体,该突变体表现为16℃可育,23℃半不育,27℃完全不育。通过图位克隆,发现该突变体突变基因为a tms1,为单碱基突变,其编码一个脂酰辅酶A合成酶。采用HPLC测定该酶活性发现,以16℃、23℃、27℃分别处理野生型和突变体蛋白后,野生型酶活性随温度的升高而降低,三个温度中16℃酶活性最高；突变体酶活性在三个温度处理之间无显著差别,但是其酶活性明显低于野生型。这表明,该酶在野生型体内具有一定的温敏特性,但不至于影响育性,而该酶突变后其对拟南芥的温敏育性产生重要影响。
其他文献
We even reported that nitric oxide (NO) functioned as a signal in thermotolerence.To clarified its relationship with hydrogen peroxide (H2O2), we investigated the effects of heat on three types of Ara
Galactinol synthase (GOLS), the key enzyme in the synthesis of raffinose family oligosaccharides (RFOs), catalyzes the condensation of UDP-galactose with myo-inositol to produce galactinol as the sole
Environmental stresses, such as drought and high temperature, are increasingly limiting factors for rice production, thus generating resistant engineering plants is of practical significance.Our proje
Cryptochrome 1 (CRY1) is a blue light receptor regulating photomorphogenesis and flavonoid biosynthesis in Arabidopsis.The enzyme 4-coumarate:CoA ligase (4CL) plays a key role in the biosynthesis of p
Plant cells respond to cold stress in a regulatory mechanism leading to enhanced cold acclimation accompanied by growth retardation.The C-repeat binding factor (CBF) signaling pathway plays a key role
Asparagine (Asn/N)-linked glycosylation is an important post-translational modification.Its multiple steps and consists of lipid-linked glycan biosynthesis on ER membrane, transfer of glycan to the co
GBSS1 (Granule-bound starch synthase1) controls amylose synthesis in all cereals.Previous reports on its regulation have been primarily focused on its transcriptional and post-transcriptional levels.I
By investigating on the specific field observation and breeding cultivation of an unique endangered plant (Begonia fimbristipula Hance) in Dinghushan Mountain, Guangdong Province, its cross-breeding i
Drought poses a serious threat to the sustainability of rice (Oryza sativa) yields in rain-fed agriculture.The bHLH transcription factors play important roles in the regulation of plant development an
Long-chain acyl-Coenzyme A (CoA) synthetases (LACSs) catalyze the formation of long-chain acyl-CoA which play important roles in fatty acid metabolism, including phospholipids, triacylglycerol (TAG) b