【摘 要】
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Objective: To investigate the effects of icariin (ICA) on the proliferation of pulmonary artery smooth muscle cell (PASMC) induced by endothelin-1 (ET-1), and explore its mechanisms.Methods: PASMC fro
【机 构】
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Department of pharmacology,Zunyi Medical College, 1 Xuefu W.Rd., Xinpu Dist., Zunyi, Guizhou, P.R.of
【出 处】
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第六届中国药理学会补益药药理专业委员会学术研讨会
论文部分内容阅读
Objective: To investigate the effects of icariin (ICA) on the proliferation of pulmonary artery smooth muscle cell (PASMC) induced by endothelin-1 (ET-1), and explore its mechanisms.Methods: PASMC from the pulmonary artery of SD rats were cultured by tissue explant method, The cells were divided into six groups:control group, model group (ET-1 200 nmol.L-1), ICA-L, M, H groups(ET-1 200 nmol.L-1+ICA10-8, 10-7, 10-6 mol· L-1)and solvent group (DMSO 0.1％), ET-1 were added in 2 h before ICA and established the model of PASMC proliferation, The ICA was maintained for 48 h;MTT assay and 5-bromo-2-deoxyuridine (BrdU) fluorescence labeling technology were used to evaluate the effects of ICA (10-8,10-7,10-6 mol· L-1) on ET-1 (200 nmol· L-11) induced PASMC proliferation, The apoptosis of PASMC were detected using Annexin V-FITC/PI kit.
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