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Tryptophan (Trp) is one of eight essential amino acids.The body content and metabolism of Trp are closely related with neurological disorders, HIV infections and cancers.Therefore, highly specific detection of Trp either individually or in proteins is urgently needed to study its diverse biological functions and applications.High-performance liquid chromatography (HPLC) has been extensively applied to specifically detect Trp for studying its diverse biological functions.Trp analysis following protein hydrolysis is also well-established.However, the existing methods usually require sophisticated instruments and additional pretreatments to improve the selectivity and sensitivity.And direct detection of Trp residue in intact proteins is still desirable.