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We found that the marine yeast strain W14-3 isolated from seawater of China Eastern Sea could produce riboflavin.It is interesting to observe that the marine yeast strain produced a large amount ofriboflavin only in the medium containing maltose under the conditions of vigorous shaking.The yeast strain was found to belong to Candida membranifaciens subsp.flavinogenie based on the results of routine and molecular identification.This is the first time to report that Candida membranifaciens subsp.flavinogenie W14-3 from the marine environment could produce riboflavin.