Various types of pharmaceutical formulations with drug delivery systems (DDS) have been developed to aid convenience and compliance of patients with treatment along with improvements in the efficacy and safety of DDS [1].Nano-particle (NP) DDS has been widely expected to create new pathways for tumor therapy[2].However, NP-DDS based on new drugs are rare despite the relative research have extensively been investigated.In previous studies, different NP-DDSs were selected, and in situ microscopy imaging and quantitation in single cells was performed in Shanghai Synchrotron Radiation Facility to establish a physiological pharmacokinetic model for these specific NP-DDSs on system, tissue and cellular levels.