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Method:Cell viability was determined using the MTT assay.To investigate the apoptosis mechanism induced by crocodile choline,the expression of apoptosis-related and pathway-related proteins were measured by Western blot and quantified using the Image J software.To determine the anti-tumor effects of crocodile choline,1 x 106 HepG2 cells was injected subcutaneously into the right flank of Balb/c nude mice to build the tumor xenograft models.