【摘 要】
：
The small heat shock proteins (sHSPs) are a diverse of molecular chaperones with molecular weight ranging from 12 to 43 kDa in different species.It is well
【机 构】
：
InstituteofEntomologyandMolecularBiology,ChongqingKeyLaboratoryofAnimalBiology,CollegeofLifeSciences
【出 处】
：
重庆市昆虫学会2012年学术交流及研讨会
论文部分内容阅读
The small heat shock proteins (sHSPs) are a diverse of molecular chaperones with molecular weight ranging from 12 to 43 kDa in different species.It is well established that theses proteins are crucial components of heat shock response.They also have important roles in other stress responses and in normal development.In present study,we cloned and characterized the full length cDNA of HSP23 gene from Delia antiqua,DaHSP23,by RACE PCR,quantitative real-time PCR and sequence analyses.The full-length cDNA,904 bp long,encodes 186 a.a.with a molecular mass of 20.9 kDa and a theoretical isoelectric points of 6.42.Homology analysis revealed that DaHSP23 shares more than 68% identity with known HSPs of other Diptera insects.DaHSP23 transcription is up-regulated in both summer diapauses (SD) and winter diapauses (WD) pupae with higher mRNA levels during WD than SD,while decreased when diapause was terminated.These results suggested that DaHSP23 is up-regulated in both SD and WD,and it may play an essential role in over-winterig and over-summering developmental arrest.
其他文献
目的:建立一种简便、可靠、稳定的膀胱癌原位移植瘤动物模型.方法:雌性Balb/C-nu裸鼠及T739小鼠按60mg/kg戊巴比妥钠腹腔注射麻醉,24＃静脉留置针,取出针芯,在距针尖15mm处将针
斑马鱼是一种用于研究脊椎动物胚胎学,发育遗传学和药物代谢动力学的优良的模型生物,具有其他模型动物所不具备的优点.其中研究较多的是建立斑马鱼的人类疾病动物模型.本文在
目的:通过原位移植的方法制备大鼠肝癌动物模型.方法:取大鼠肿瘤细胞Walker-256制备为浓度1×108个/ml,分别于Wistar大鼠肝包膜下接种0.04ml.观察大鼠肝脏实体瘤的生长情况及
在全球面临化石资源危机的今天，工业生物技术的重要作用及其发展潜力日益凸现，作为工业生物技术的核心技术，生物催化与生物转化技术得到科学界和产业界的空前关注。生物催化
Anopheles aconitus is a well-known vector of malaria and is broadly distributed in the Oriental Region,yet there is no information on its population genetic
To gain a better understanding of the molecular mechanisms regulating pupal diapause of the onion maggot Delia antiqua,PCR-based suppressive subtractive hyb
Malaria persists today as the most widespread and devastating protozoan disease of humans.Over 3 billion people live under the threat of malaria and it kill
Twenty-three species and two subspecies of the genus Ancistrocerus Wesmael are taxonomically reviewed.Three new record species of this genus from China are
Insect immune responses are precisely regulated to maintain immune balance.Although in locust immune responses and regulatory mechanisms have been well stud