【摘 要】
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The rabbit is a common animal model that has been employed in studies on various humandisorders,and the generation of genetically modified rabbit lines is highly desirable.Female rabbitshave been succ
【出 处】
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华东地区第十四届实验动物科学学术交流会
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The rabbit is a common animal model that has been employed in studies on various humandisorders,and the generation of genetically modified rabbit lines is highly desirable.Female rabbitshave been successfully cloned from cumulus cells,and the somatic cell nuclear transfer (SCNT)technology is well established.The present study generated hypoxanthine phosphoribosyltransferase(HPRT) gene knockout rabbits using recombinant adeno-associated virus-mediated homologousrecombination and SCNT.Gene trap strategies were employed to enhance the gene targeting rates.
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