Posttranslational modifications (PTMs) of the microtubule cytoskeleton play significant roles in all stages of embryogenesis and they are critically important for the formation and maintenance of meiotic and mitotic spindles to prevent aneuploidies and for cellular differentiation during pre-implantation embryo development.Acetylation and detyrosination/tyrosination are among the PTMs that modulate microtubule functions and provide readable codes for selective association with microtubule motor proteins to translocate specific cellular components to their functional destinations.