探讨和研究新理论和新机理,对于分析化学学科的发展具有重要意义,同时可以为分析化学的未来发展指引新方向。近年来,光电化学适配体传感技术作为一种高效的分析手段,已经迅速发展成为研究者的研究热点[1,2]。然而由于其较短的发展时间,光电化学适配体传感技术仍处于初始阶段且尚存在许多不完善的领域如传感机理的探讨。在光电化学适配体传感领域,现有的传感机理主要有光电化学氧化机理和位阻效应机理。尽管这两种经典的理
苯胺和对苯二胺作为染料制备过程中的重要中间体,与我们的生活息息相关[1]。如果皮肤在短期内吸收大量苯胺会使人出现高铁血红蛋白血症,而长期低浓度接触苯胺可引起中毒性肝病。对苯二胺目前被允许在染发剂中使用,但使用量有明确的限制[2]。因此,建立一种快速简便的方法实现对苯胺和对苯二胺的检测非常有必要。
目的:组合使用重组人工角膜模型EpiOcularTM EIT试验和牛角膜浑浊和渗透试验(BCOP)两种动物替代体外模型评估核桃青皮色素的眼刺激性。方法:BCOP试验:采用屠宰场获得的新鲜牛眼,将新鲜分离的角膜水平固定于支架。封闭角膜将支架分为上下2个隔室,试验时将色素配制成20%悬液,取750 μL暴露于角膜表面4h后通过测量角膜的透光度变化评估浑浊度。往上隔室添加荧光素钠孵育90min后检测下隔
In this paper,we described a label-free and universal platform based on microchip electrophoresis(MCE)for rapid,automatic detection of antibiotics,with chloramphenicol(CAP)as model.Firstly,CAP was cap
The arrangement of essential amino acids in catalytic sites of native enzymes are important in nature inspired catalyst designs.The remarkable catalytic properties of enzymes is likely to bring to the
An artificial enzyme was constructed by attaching short peptides with active sites(SHELKLKLKL,WLKLKLKL)onto carbon nanotubes(CNT).It was found that the combination of SHE amino acids was essential to
polycyclic aromatic hydrocarbons(PAHs)are a kind of important procarcinogens.Formation of hydroxy polycyclic aromatic hydrocarbons(OH-PAHs)from PAHs via phase Ⅰ metabolism was reported to be the key p
Superhydrophobic surface based microarrays,which are applied to many fields including cell arrays and piptide/protein arrays,liquid transfer chips,diagnose devices,et.al.have attracted a great deal of
Due to the low concentration of analytes and the complexity of the sample matrix,efficient extraction technique is essential for reliable and accurate analysis.On the other hand,Surface enhanced Raman