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  5. Establishment of Loop-Mediated Isothermal Amplification Technique for Rapid Detection of Bluetongue

Establishment of Loop-Mediated Isothermal Amplification Technique for Rapid Detection of Bluetongue

来源 :2016华南实验动物科学国际研讨会、第三届实验小型猪国际论坛暨广东·九州实验动物学术交流会 | 被引量 : 0次 | 上传用户：lifei111
【摘 要】
：
Bluetongue disese is one of the major domestic and wild ruminant viral diseases and has caused significant economic loss to cattle and sheep producers.In this study,a Loop-mediated isothermal amplific
【作 者】
：
Jun Liu Zhen-zhen Qiu Chang-fu Ji Hui-qiong Yin Hong-wei Li Jin-gang Zhang Wei-wang Gu 
【机 构】
：
Department of Laboratory Animal Center, Southern Medical University, Guangzhou, 510515;School of Bio
【出 处】
：
2016华南实验动物科学国际研讨会、第三届实验小型猪国际论坛暨广东·九州实验动物学术交流会
【发表日期】
：
2016年10期
【关键词】
：
Bluetongue virus LAMP rapid detection NS1 gene 
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　　Bluetongue disese is one of the major domestic and wild ruminant viral diseases and has caused significant economic loss to cattle and sheep producers.In this study,a Loop-mediated isothermal amplification (LAMP) assay was developed for rapid,sensitive and specific detection of Bluetongue virus.Four primers (two inner primers,two outer primers) were designed specifically to recognize the NS1 gene of Bluetongue virus.After optimization,target gene was amplified and detected within 30 min under 62℃ isothermal conditions.
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