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The enrichment of circulating tumor cells (CTCs) from a pool of blood cells with specificity and sufficient sensitivity is very important for cancer diagnosis and treatment.In this study,a thin sheet of hyaluronic acid (HA)-functionalized electrospun polylactic-co-glycolic acid (PLGA) nanofibers was integrated into a microfluidic platform for CTCs capture.A facile approach was developed to immobilize HA onto the electrospun PLGA nanofibers via amino and carboxylic condensation reaction through polyethyleneimine (PEI) linkers.