Fungi play potentially crucial roles in nutrient and carbon cycling and interact with other organisms,thereby influencing food web dynamics.And occurrence of fungi in water supply to using in water tap in houses or public system may have many of health problems,as cancer diseases infection,or respiratory problems by taking shower and inhalation spores from water.This study aim to investigated occurrence and frequencies of fungi in drinking water by using two kinds of media MEA and SDA and quantification real-time PCR,and detection mycotoxigenic fungi.