Aim Adipose tissue releases adipokines that play important roles in metabolic and cardiocerebrovascular homeostasis.This study was to discover novel adipokines using caloric restriction model.Methods Adipokine candidates were captured by gene array and bioinformatics analysis and verified by preparation of recombinant protein and antibody.Results We established a potential secreted protein database containing 208 genes and identified a novel adipokine, Subfatin, that was the highest expressed in subcutaneous fat of both rodents and humans among 15 detected tissues.The secreted mammalian Subfatin was a glycosylated protein.Subfatin was located diffusely throughout the adipose tissue except lipid droplets, with comparable expression between adipocytes and strormal cells, but much lower expression in macrophages than adipocytes.Subfatin was downregulated in white adipose tissue of caloric restriction rats, whereas dramatically upregulated during white adipocyte differentiation as well as in white adipose tissue of dietinduced obese mice.Subfatin was annotated as Meteorinlike (Metrnl) in public databases, a similar transcript of Meteorin (Metrn, also known as glial cell differentiation regulator).Meteorin displayed a brainspecific expression and was scarce in various adipose tissues, in contrast to the tissue expression patterns of Subfatin.Conclusions Subfatin is a novel adipokine regulated by adipogenesis and obesity, with tissue distribution different from its homologue Meteorin.