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The integration of ultra high-performance liquid chromatography(UHPLC),quadrupole-time of flight-mass spectrometry(Q-TOF-MS),solid phase extraction(SPE),and nuclear magnetic resonance(NMR)is a powerful platform for the identification of components in complex system,for instance,bioactive compounds in herbal medicines,biomarkers in animal biofluids,nutrient contents in food and toxic components in environmental samples.