Introduction: ASCs have multiple lineage differentiation ability and can be used to generate adipose, bone,cartilaginous, muscle, neural and vascular tissues.However,the potential of ASCs to differentiate towards urothelial phenotype and the related mechanisms remain obscure.Experimental: ASCs were isolated from the human adipose tissue of individuals undergoing liposuction procedures.Labeling with CM-DiI, the ASCs were mixed with an immortalized urothelium cell line (HUC) and implanted into the subcutaneous tissue of athymic mice for 4 weeks.In vitro, ASCs were induced by conditioned media (CM) and the transwell co-culture with HUC for 21 days.Protein and mRNA expression of the urothelium specific markers UP1A and UP-II were detected.Array detection of the upper medium was used to screen 41 cytokines and receptors in induced ASCs at different time points.