By using publicly available expression profiling data in lung cancer and integrating bioinformatics analyses, we screened out a H3K27-acetylation-activated LINC01116,whose expression is significantly increased and is correlated with outcomes in nonsmall cell lung cancer (NSCLC).RNA-seq analysis revealed that LINC01116 knockdown preferentially affected genes that are linked to antitumor immune responses-related genes.Mechanistic investigations found that LINC01116 serves as a scaffold for two distinct epigenetic methylation modification complexes (PRC2 and DNMT1) and epigenetically modulates transcription of IRF7 and CD1D in nucleus.And knockdown of LINC01116 promoted the cytotoxicity of NKT cells via epigenetic-mediated upregulating CD1D expression on NSCLC cells.