Polyhydroxybutyrate (PHB) production with Cupriavidus sp.CY-1 using various toxic compounds PhaC gen

来源 :第六届亚洲特大城市环境与公共健康论坛暨中国环境科学学会环境医学与健康分会2015年会 | 被引量 : 0次 | 上传用户:flywhc
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  Different types of toxic compounds were converted into polyhydroxybutyrate (PHB) using the isolated bacteria Cupriavidus sp.CY-1 in the present study.Tween-80 influence on toxic compounds degradation ability of CY-1 were analyzed using high pressure liquid chromatography.CY-1 showed highest removal of naphthalene (100±6%), phenol (96±7%), and lowest removal of alkylphenols without Tween-80 addition.However, Tween-80 addition enhanced the degradation capacity of CY-1, and showed highest removal of 4-tertiary-butylphebol (74±5%), followed by phenol (69±5%), 4-chlorophenol (59±3%),4-tertiary-octylphenol (53±5%), and naphthalene (48±5%).Further experiments were carried out for conversion of toxic compounds into PHB.CY-1 grown with phenol (48±6%) and naphthalene (42±4%) showed highest PHB production.The functional groups, structure and thermal properties of the produced PHB were analyzed.In addition the expression of PhaC gene was quantified at transcriptional level through real time quantitative PCR.The maximum PhaC transcript expression was 5.37 folds at 100 mg/1 nitrogen concentration.
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