【摘 要】
：
目的 研究美沙拉嗪、蒙脱石散及美沙拉嗪联合蒙脱石散对2,4,6-三硝基苯磺酸(TNBS)诱导的实验性结肠炎大鼠肠组织中增殖细胞核抗原(PCNA)、血管内皮细胞生长因子(VEGF)表达的影响.方法 在建立大鼠实验性结肠炎模型的基础上,分为模型组、美沙拉嗪组、蒙脱石散组、美沙拉嗪联合蒙脱石散组,各干预组每日灌服相应剂量药物.并设正常组.于灌胃满12d时处死大鼠,取结肠标本,采用免疫组织化学方法检测肠组
【机 构】
：
650032 昆明医科大学第一附属医院儿科 650032 昆明医科大学第一附属医院病理科
【出 处】
：
The 14th Congress of Gastroenterology China(第十四届全国消化系病学术会议)
论文部分内容阅读
目的 研究美沙拉嗪、蒙脱石散及美沙拉嗪联合蒙脱石散对2,4,6-三硝基苯磺酸(TNBS)诱导的实验性结肠炎大鼠肠组织中增殖细胞核抗原(PCNA)、血管内皮细胞生长因子(VEGF)表达的影响.方法 在建立大鼠实验性结肠炎模型的基础上,分为模型组、美沙拉嗪组、蒙脱石散组、美沙拉嗪联合蒙脱石散组,各干预组每日灌服相应剂量药物.并设正常组.于灌胃满12d时处死大鼠,取结肠标本,采用免疫组织化学方法检测肠组织VEGF和PCNA表达阳性细胞率,采用荧光定量PCR检测肠组织中VEGF和PCNA的基因表达情况.结果 在免疫组织化学检测中,与正常组相比,模型组肠组织中PCNA降低,差异有统计学意义(P＜0.01)；美沙拉嗪联合蒙脱石散组与正常组比较差异无统计学意义(P=0.133)；3组干预组与模型组比较,肠组织中PCNA的阳性表达率均升高,差异均有统计学意义(P均＜0.01)；3组干预组间两两比较差异均无统计学意义(P均＞0.05).在免疫组织化学检测中,与正常组相比,模型组肠组织中VEGF明显增高,差异有统计学意义(P＜0.01)；美沙拉嗪联合蒙脱石散组与正常组比较差异无统计学意义(P=0.746)；3个干预组分别与模型组比较差异均有统计学意义(P均＜0.05)；美沙拉嗪联合蒙脱石散组的VEGF阳性表达率低于美沙拉嗪组,美沙拉嗪组明显低于蒙脱石散组(P均＜0.05).在荧光定量PCR检测中,与正常组相比,模型组肠组织中PCNA降低,差异有统计学意义(P=0.047)；3个干预组与模型组相比差异均无统计学意义(P均＞0.05)；3组干预组间两两比较差异均无统计学意义(P均＞0.05).在荧光定量PCR检测中,与正常组相比,模型组肠组织中VEGF明显增高,差异有统计学意义(P=0.002)；美沙拉嗪组和美沙拉嗪联合蒙脱石散组与模型组比较,差异均有统计学意义(P均＜0.05)；美沙拉嗪组与蒙脱石散组相比差异无统计学意义(P=0.556)；蒙脱石散组及美沙拉嗪组与美沙拉嗪联合蒙脱石散组相比差异均有统计学意义(P均＜0.05).结论 美沙拉嗪与蒙脱石散联合治疗能促进肠上皮细胞增殖,蒙脱石散能辅助美沙拉嗪促进溃疡愈合,降低血管通透性,效果优于单独使用美沙拉嗪.
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