Arbuscular mycorrhizal(AM)is a mutualistic symbiosis wildly formed between AM fungi and the roots of terrestrial plants.Citrus have rare or short root hairs and thus are fairly depends on AM symbiosis for nutrient uptake.To unravel genes involved in AM symbiosis in roots of a frequently used citrus rootstock Poncirus trifoliata(L.)Raf,we conducted transcriptomic(RNA-Seq)and iTRAQ-based proteomic analysis using the roots infected or uninfected with AM fungus Glomus versiforme.A total of 32 million sequencing read pairs produced by RNA-seq were unambiguously mapped on citrus and AM fungus genomes,led to 282 genes differentially expressed in mycorrhizal roots and 896 AM fungus genes detected with three biological replicates.The transcription expression of 132 up-regulated genes was further confirmed by quantitative RT-PCR.Ninety-three genes were found conserved in mycorrhizal symbiosis by the comparison with previous reported medicago transcriptomic data.