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A comparative transcriptomic analysis was designed to search for novel genes influencing clavulanic acid (CA) biosynthesis in Streptomyces clavuligerus.Besides the wild type strain (WT, S.clavuligerus ATCC27064), four mutants with different CA production, M1 to M4, were selected for comparative transcriptomic analysis.The descending order of CA production is M1>M2>WT>M3>M4.