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Herbicides have been extensively used as selective emergence herbicides in the control of weeds on agriculture, and they are highly toxic when the content reaches a dangerous level.Here we developed an integrated system combining microfluidic device with electrospray ionization quadrupole time-of-flight mass spectrometer (ESI-Q-TOF-MS) for detecting a series of herbicides on a single C30 bead, which realized the rapid detection, low consumption, high sensitivity, and accurate definition.