论文部分内容阅读
The distribution of genetic diversity ofToxoplasma gondii in wildlife is of interest to understand the transmission of this parasite in the environment.Limited information on T.gondii genotypes has been reported in wildlife in China.Using PCR and multilocous polymerase chain reaction:restriction fragment length polymo rphism (PCR:RFLP) technology,we conducted a survey to examine genetic diversity of this parasite in wildlife on the Qinghai:Tibet Plateau,China.In total,183 brain tissues of different wild animals,including 48 Qinghai vole (Microtus fuscus),101 Plateau Pika (Ochotona curzoniae) and 34 Tibetan Ground:tit (Pseudopodoces hum ilis),were tested for T.gondii infection.11 of which were found to be positive for the T.gondii B 1 gene by PCR amplification.These positive DNA samples were typed at 10 genetic markers,including 9 nuclear loci (SAG 1,5:and 3:SAG2,alternative SAG2,BTUB,GRA6,L358,PK1,c22:8,c29:2),and an apicoplast locus Apico.Six were successfully genotyped at eight or more genetic loci,and were grouped to three distinct genotypes.Fo ur samples belonged to ToxoDB Genotype #10 and the other two samples were identified as two new genotypes (http://toxodb.org/toxo/).The results show that there is a potential risk for the transmission of this parasite thro ugh the wildlife in this region.To our knowledge,this is the ?rst report of genetic typing of T.gondii isolates in wildlife on the Qinghai:Tibet Plateau,China.