Epigenetic modification has an important role in fertilization and proper embryonic development,and several human diseases are associated with epigenetic modification disorders.However,the dynamics and functions of long non-coding RNAs (IncRNAs),one type of epigenetic regulator,in human pre-implantation development have not yet been demonstrated.In this study,a comprehensive analysis of human and mouse early-stage embryonic IncRNAs was performed based on public single-cell RNA sequencing data.Expression profile analysis revealed that IncRNAs are expressed in a developmental stage-specific manner during human early-stage embryonic development,whereas a more temporal-specific expression pattern was identified in mouse embryos.Weighted gene co-expression network analysis suggested that IncRNAs involved in human early-stage embryonic development are associated with several important functions and processes,such as oocyte maturation,zygotic genome activation and mitochondrial functions.We also found that the network of IncRNAs involved in zygotic genome activation was particularly preserved between human and mouse embryos,whereas in other stages there was not a one-to-one correlation between human and mouse embryo IncRNAs.This study provides insight into the molecular mechanism underlying IncRNA involvement in human pre-implantation embryonic development.