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Objective:To investigate the feasibility of tissue engineered skins with epidermal stem cells and amniotic membrane for diabetic impaired wound.Methods:Epidermal stem cells (ESCs) derived from SD rats were isolated, cultured, identified and labled with 5-bromo-2-deoxyuridine (BrdU) in vitro.The wound model of diabetic SD rats were established, and then divided into A, B, C group.Tissue engineered skins with labled BrdU ESCs and amniotic membrane were implanted to group A.Amniotic membrane were implanted to group B.Group C as control received no cells or amniotic membrane.At 7,14 days after transplantation, general situation of wound healing, the healing rate of wound, hematoxylin and eosin (HE) staining, immunohistochemical staining of Brdu and proliferating cell nuclear antigen (PCNA)in wounds were investigated.Integral optical density mean of positive cells were measured with image analysis software.Results:Compared with the other two groups, the healing time of group A was faster.And the wound healing rate in group A was significantly higher at different observation times (P<0.01).BrdU-positive cells in the wounds and newborn epidermis of group A were visible, while wound tissue of the other two groups had no BrdU-positive cells.PCNA-positive cells in wound tissue of each group could be seen, but integral optical density mean of positive cells were significantly different in group A compared to that in group B, C (P<0.01).Conclusion :Tissue engineered skins with epidermal stem cells and amniotic membrane may contribute to healing of diabetic impaired wound.