The type Ⅲ secretion system (T3SS), encoded by hrp (hypersensitive response and pathogenicity) genes in Gram-negative phytopathogenic bacteria, delivers repertoires of T3SS effectors (T3SEs) into plant cells to trigger the hypersensitive response (HR) in nonhost or resistant host plants and promote pathogenicity in susceptible plants.The expression of hrp genes in Xanthomonas is regulated by two key regulatory proteins, HrpG and HrpX.However, the interactions between hrp gene products in directing secretion are largely unknown.Here we demonstrated that HrcT of X.oryzae pv.oryzicola (Xoc) functions as a T3S component and positively regulates the expression of hrpX.Transcription of hrcT occurs via two distinct promoters, one is with the hrpB operon (T1) and the second (T3) within hrpB7.Via either T1 or T3 promoter, the defect in Hrp phenotype by hrcT mutant was restored in the presence of hrcT only from Xanthomonas species, but not other phytopathogenic bacteria.An N-terminally truncated HrcT was able to bind the hrpX promoter and activate the expression of hrpX, supporting that HrcT is a positive regulator of hrpX.A revised model showing the regulatory interactions between HrcT, HrpX and HrpG is proposed.