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Objectives To evaluate acute myocardial ischaemia using 11C-N-CH3-Dopamine which is cardiac sympathetic nerve molecular probe compared with 13NH3H2O myocardial perfusion.Methods Bama miniature pigs (n =12, 6 male, 6 female, 1-1.5 y old, 30 kg-40 kg, provided by the Animal Research Institute of Northeast Agricultural University of China) were used in this study.