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An aerobic, Gram-negative, milky-pigmented, rod-shaped bacterial strain DDT-3T, isolated from a DDT-contaminated soil in Liyang, China, was subjected to a polyphasie taxonomic investigation.Strain DDT-3T grew at a temperature of 20-40℃ (optimum at 30-37℃) and pH range of 5.0-10.0 (optimum at pH 7.0-8.0).The G+C content of the total DNA was 70.1 mol%.The 16S rRNA gene sequence of strain DDT-3T showed the highest similarity to that of Pseudoxanthobacter soli CC4T (99.64%), followed by Kaistia dalseonensis B6-8T (93.31%), Kaistia soli 5YN9-8T (93.06%) and Amorphous orientalis YIM D10 T (93.00%).Strain DDT-3T showed less than 92.60% similarities with other species of the family Xanthobacteraceae.The major cellular fatty acids of strain DDT-3T were C19:0 cyclo ω8c (42.58%), C16:0 (33.21%) and C18:1 ω7c (10.04%).The major respiratory quinone was ubiquinone Q-10 (100%) and the major polar lipids were glycolipid, lipid, phosphatidylcholin, aminolipid,phosphatidylmethylethanolamin, diphosphatidylglycerol and phosphatidylglycerol.